Molecular Pathology 2025 (1 Hour)

Author: Anderson Continuing Education
Publisher: Anderson Continuing Education
Date Published: 2025
Pages:
Cover Type: Soft Cover
Expiration Date: 12/31/2027
Level of Difficulty: Basic

Content Objectives
Nucleic Acid Hybridization Methods
  • Specify the principle on which nucleic acid hybridization methods are based.
  • Recognize the function of a “reporter” molecule in nucleic acid hybridization tests.
  • Identify how the degree of stringency in a nucleic acid hybridization test influences probe–target hybridization.
  • Name the hybridization format that uses the patient’s cells or tissues as the solid support phase to allow pathogen identification.
  • Recognize the principle behind polymerase chain reaction (PCR).
  • Define amplicon.
  • Specify the advantages of nested PCR.
  • Recognize the reasons why real-time PCR automated instruments are noteworthy.
  • Identify applications for melting curve analysis (Tm) of the target DNA in real-time PCR assays.
Amplification Methods: Non-Polymerase Chain Reaction-Based
  • Name a testing application using Nicking Endonuclease Amplification. 
Postamplification End-Point Analysis
  • Specify a nucleic acid sequencing method that enables the sequencing of many strands of DNA simultaneously. 
Characterization of Microorganisms Beyond Identification 
  • Identify the molecular method used for microbial strain typing which is often referred to as the “gold standard” for microbial typing. 

 

It meets the Florida one-hour specialty requirement in molecular pathology. For those certified by ASCP, this course provides one contact hour in molecular pathology.

"Nucleic Acid-Based Analytic Methods for Microbial Identification and Characterization" introduces molecular techniques used in diagnostic microbiology.

Topics:

  • Nucleic acid hybridization
  • Polymerase chain reaction (PCR)
  • Real-time PCR
  • Next-generation sequencing (NGS)
  • Pulsed-field gel electrophoresis (PFGE)
“Nucleic Acid-Based Analytic Methods for Microbial Identification and Characterization,” by Patricia M. Tille, PhD, MLS(ASCP), AHI(AMT), FACSc. Chapter 8 of Bailey & Scott's Diagnostic Microbiology, 16th Edition, pages 122-151. Published by Elsevier in 2026.

NameDeliveryCourse CodeHoursPrice 
Reading Material and QuizShipped1MOLECPATH251.0$16.00
Reading Material and QuizOnline1MOLECPATH25 (Online)1.0$16.00
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Reading Material and Quiz
(1MOLECPATH25)
$16.00
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1.0
Reading Material and Quiz
(1MOLECPATH25 (Online))
$16.00
Online
1.0